GATA3 Antibody

Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

Gata-3 Monoclonal Antibody (TWAJ), PerCP-eFluor 710, eBioscience™

Catalog Number:

46-9966-42

The general aim of the research was to characterize innate lymphoid cells 2 (ILC2). The specific goals of the experiment was to examine GATA3 expression from ILC2 population from mouse large intestines. The reason for selecting this product was because it was recommended by colleagues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lamina propria of large intestine from mouse

Primary Incubation

1:20 (2ul of antibody in 40ul of sample)

Blocking Agent

1X Permeabilization buffer (ThermoFisher)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PerCP-eFluor 710

Results Summary

The quality is good enough to detect GATA3 expression from ILC2.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

High quality

The Bad

Cost

The Bottom Line

For detection, the staining buffer set is required.(Transcription Factor Fixation/Permeabilization Concentrate and Diluent, Cat# 00-5521-00, ThermoFisher)

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