Good Antibody For Human BDCA2(CD303a) Staining

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen Antibodies

Product Name:

CD303a Monoclonal Antibody (201A), APC, eBioscience™

Catalog Number:

17-9818-41

I used this antibody to characterize human plasmacytoid and conventional dendritic cells in the thymus. pDCs can be defined as CD11c-CD123hiCD13-BDCA2+ subset and cDCs can be defined as CD11c+CD123loCD13+BDCA2- subset. CD303a aliases BDCA2. This antibody worked well and gave a nice distinct population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human thymocytes

Primary Incubation

1:100 for 2h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Ease of optimization

The Bad

Expensive

The Bottom Line

Good quality antibody and worth buying it.

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