Invitrogen Antibodies by Thermo Fisher Scientific
Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
A-11013
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Our research is the molecular imaging of diseased tissues. In this experiment, we examined the expression of VEGFR-1 and CD31 in HCC4006 and A549 tissues. We used this product as the secondary antibody for detection of VEGFR-1. We selected this product because we trust the company.
Immunofluorescence
Human cancer cell lines H4006 and A549
1:200, 12 h, 4 degree C
Odyssey blocking solution
1:10,000, 3 h, 23 degree C
None
Fluorescence imaging system (Odyssey)
We were able to detect VEGFR-1 expression in the tissue, which corresponded to CD31 expression found on vasculature.
N/A
Great detection, simple to use, affordable
This is an excellent secondary antibody that would work for any human antibodies.