Good Antisera To Detect Human Neutrophil Elastase

Detection of neutrophil elastase (NE) is important in the studies of inflammation, specifically in studying the inflammatory responses of neutrophils. NE is a powerful serine protease that is expressed mainly by neutrophils, which release NE upon stimulation. Specific NE activity is measured by a peptide cleavage assay, but it is also important to know how much of total NE has been released by neutrophils under specific conditions. Total NE could be determined by western blotting in comparison with the known quantities of purified NE. We used this rabbit antiserum to detect NE by western blotting.