PE Anti-Mouse F4/80 Antibody For Flow Cytometry

September 07, 2017

Pathology
Stanford University
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse F4/80 Antibody

Catalog Number:

123109

I am interested in characterizing macrophage populations in the murine colonic lamina propria in the context of colon cancer. Therefore, we optimized the stain for F4/80.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse colon tissue sample

Primary Incubation

1:200 dilution; 20 min at 4 deg C

Blocking Agent

FcBlock for 5 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

F4/80 positive cells are clearly seen

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear stain

The Bad

None

The Bottom Line

This antibody is good

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