Abcam
Anti-ATF4 antibody
ab184909
Browse Similar Products: ATF4 Western Blot Antibodies
PERK is a key protein that is phosphorylated and activated during ER stress, responsible for the phosphorylation eIF2alpha which then activates ATF4. Determining the amount of ATF4 present due to a variety of stress conditions is helpful in assessing the severity of ER stress.
Western Blot
Total lysates of H4IIE rat hepatoma cells
1:500 in 1:3 odyssey block:1X TBST (0.1% Tween) shaking, overnight at 4 degrees C
1:3 odyssey block:1X TBS at room temperature for 1 hr.
1:15000 Goat anti-rabbit IRDye in 1:3 odyssey block:1X TBST (0.1% Tween) and 0.2% SDS for one hour at room temperature in the dark
N/A
Licor Odyssey Clx
This antibody works and appears around 38kDa, just like it is predicted. However, we attempted to use a blocking peptide for this specific antibody and that did not work. Therefore I am concerned this visible band may be non-specific binding.
Shows up...
May not be the correct protein
Order this antibody only if you can confirm with a blocking peptide this is the correct band.