Leinco Technologies
Goat Anti-Rabbit IgG (H&L) DyLight® 488 Secondary Antibody Adsorbed Against: Hu.
R1321
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This secondary antibody was used to perform indirect immunofluorescence microscopy on HeLa cells to detect the ER/mitochondria. It showed strong immunoreactivity that was consistent mitochondria. Background levels were low. This antibody is a good choice for immunofluorescent microscopy.
Immunofluorescence
HeLa cells fixed with 3.7% paraformaldehyde
1 hour at room temperature in blocking agent at 1:300
PBS in 1% normal goat serum and 0.1% triton x-100
1 hour at room temperature (1:300)
DAPI
Confocal microscopy
Bright and specific anti-Rabbit secondary. Cheaper than competitors!
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Strong, specific and cheap
None
Great secondary for half the price