Time Course Effect Of Chemoattractant On UCP2 Expression

August 30, 2017

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

UCP2 (D1O5V)

Catalog Number:

89326

UCP2 is a inner mitochondrial membrane protein transport protein. It inhibits mitochondrial glucose oxidation and promotes glycolysis in human pluripotent stem cells. Here we checked the time course effect of UCP2 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human lung cells

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% BSA prepared in 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We observed time dependent increase of UCP2 expression in human lung cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This antibody is very good for western blot experiments.

The Bad

We have to use at least 1:1000 dilution otherwise the signal is very weak.

The Bottom Line

This antibody is very good for western blot. Highly recommended.

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