LRP6 Antibody for Western Blotting

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

LRP6 Rabbit mAb

Catalog Number:

3395

LRP6 antibody from Cell Signaling is an excellent detector of the target protein. Antibody can be used on lysates from a wide variety of cell lines or patient samples.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human ovarian cancer cells

Primary Incubation

1:1000 dilution overnight at 4 degrees on shaker

Blocking Agent

5% BSA in TBS-T

Secondary Incubation

1:2000 Rabbit secondary (BioRad), 1 hour incubation at room temperature on shaker

Tertiary Incubation

N/A

Detection

Pierce ECL Western Blotting Substrate

Results Summary

Compared to first lane with untreated control, LRP6 expression was reduced in other three lanes representing cells with three different drug treatments, as expected.

DOI or PMID #

27888804

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great specific antibody for LRP6

The Bad

N/A

The Bottom Line

This is an excellent antibody for detecting LRP6 in many different cell types.

Share your experience with other scientists. Write a Review! »

Join the discussion