Brg1 Staining In Mouse Pancreatic Cryosection

August 24, 2017

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

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Quality of Results

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Company:

R&D Systems

Product Name:

Human Brg1 Antibody

Catalog Number:

AF5738

Our goal is to evaluate the contribution the Brg1 ATPase subunit of the Swi/Snf chromatin remodeling complex has in pancreatic islets. Staining mouse pancreas cryosections with the goat polyclonal antibody from R & D Systems (AF5738, 1:500) revealed that Brg1 is expressed in all pancreatic tissues, however the staining pattern in islets appears more robust than in exocrine tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse pancreas cryosection

Primary Incubation

1:500, Overnight, 4 Deg Celcius

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS

Secondary Incubation

1:1000, Donkey anti-Goat Cy3

Tertiary Incubation

N/A

Detection

Immunofluorescence

Results Summary

Antibody works excellent in mouse cryosections. 1:500

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Excellent detection at 1:500

The Bad

N/A

The Bottom Line

Excellent detection at 1:500 in mouse cryosections

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