Promega
GloMax 20/20 Luminometer
E5311
Info:Supplier Page View Product Specs View Product
The GloMax 20/20 Luminometer is a great machine for evaluating luminescence via reporter assays. It is very easy to use and generates results quickly.
Reporter Assays
Human cancer cells
Cancer cells can be transfected with desired luciferase-tagged reporters. Reagents such as the Dual-Glo Luciferase Assay reagents can be added to the cells, transferred to eppendorf tubes, and read on machine.
One should perform a standard curve with cell numbers to make sure machine is accurate with readings
The output from machine is raw counts. Raw counts can be compared to control (such as treated vs. untreated controls) to generate percentages of effect or relative luciferase activity (quotient of firefly to renilla luciferase).
Touchscreen capability, 96-well formats are also available in other versions
N/A
Very user friendly
None to note
The GloMax luminometer is a machine with high sensitivity to luminescence and is a great tool for detecting reporter activity.