Tonbo Biosciences
RedFluor 710 anti-mouse CD3
80-0032-U100
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I am studying recognition of viruses in mouse macrophages during infection with RNA viruses. For this experiment, I was determining the cellular composition of spleens following infection with West Nile virus by flow cytometry. I was testing to see if a specific Pattern Recognition Receptor was required for macrophage polarization during infection. For this experiment, I needed a CD3 antibody that I could use as part of my panel to gate out CD3 positive cells. I chose this CD3 antibody from Tonbo because it was in the correct color (the 700 channel) and I had good success with Tonbo antibodies in the past. They offered the clone, the color, and the amount of antibody that I was looking for at a reasonable price. Their shipping was fast and reliable, so the antibody was in the lab when I needed it.
Flow Cytometry
Mouse Splenocytes
1 hour 4C on live cells 1 uL/100 uL of cells (~1e6)
N/A
LSRII Flow cytometer
Compared to the isotype control and unstained controls there is a clear shift of the CD3+ population.
Easy to use, clear separation between negative and positive populations
No Issues
Tonbo has reliable antibodies in a variety of fluorochromes.