ATPlite Cytotoxicity Assays

UAB
Radiation Oncology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Revvity

Product Name:

ATPlite Luminescence Assay System

Catalog Number:

6016949

The ATPlite Luminescence Assay System is very easy to use as it involves two simple steps to determine cell viability based on amount of ATP present. Little optimization is required for usage of this product.

Experimental Design and Results Summary

Application

Cell viability assays with luminescence

Starting Material

Human breast cancer cells

Protocol Overview

Half of recommended lysis buffer may be added to lyse cells and release ATP. Half of recommended substrate reagent may be added to determine cell viability. Cell viaiblity was determined following addition of cytotoxic drugs/agents.

Tips

It is important to test compound interference with luminescence in order to obtain accurate results.

Results Summary

Cell viability is determined by comparison of ATP content of treated cells with untreated cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very easy to use

The Bad

Possible interference of agents with reagents.

The Bottom Line

Great easy-to-use assay to determine cell viability and can be used for many cell lines, patient samples, and agents

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