R&D Systems
Anti His Antibody
MAB060
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We are interested in site specific labeling of antibody and use his tagged. At the end, we perform western blot to confirm that isolated and purified proteins are still with his tagged or not. The reason we selected the product is because we can reuse it and a very low concentration required.
Western Blot
Engineered antibody
With Anti his antibody for 1 hr
2% skimmed milk
Anti mouse HRP antibody
None
Chemi luminescence
Clearly visible his tagged protein
N/A
Very low conc. required
Nothing so far
Best for western blot