System For Kinetic And Affinity Analysis

University of Pittsburgh
Medicine
Postdoctoral Research Associate

Overall

Performance

Ease-of-Optimization

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Company:

GE Life Sciences

Product Name:

Biacore X100 Surface Plasmon Resonance System

Catalog Number:

BR110073

Our lab is interested in small molecules and antibody fragments to develop novel tracers for cancer imaging. The most important part is to access the affinity of these molecules. We use BIACORE X100 on the daily basis to evaluate the affinity of these molecules as primary selection criteria. This is the best SPR instrument and at the same time, we can use several molecules for analysis.

Experimental Design and Results Summary

Application

Kinetics and affinity

Starting Material

Antibody fragments

Protocol Overview

Immobilization of PDL-1 ligand on the CM5 chip and throwing the different concentration of analyte to evaluate on and off rate of the analyte

Tips

Use nM conc. of analyte

Results Summary

Analyte with high affinity gives up to 2000 RU with nM to pM affinity

Features Summary

Response unit is depend upon the affinity of different analyte

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Its a quick and fully automatic

The Bad

I wish I could load more samples at the same time

The Bottom Line

Simple to use

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