BD Biosciences
BV711 Rat Anti-Mouse TNF Clone MP6-XT22
563944
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Browse Similar Products: TNF Flow Cytometry Antibodies
My main goal was to validate if the antibody was working so I did a very simple experiment of in vitro T cell stimulation. P14 splenocytes (mouse transgenic T cells) were activated in vitro with Gp33 peptide for 24hour in the presence of BFA and stained for intracellular cytokine production.
Flow Cytometry
Mouse splenocytes
30min
N/A
LSRII
Good TNF staining
Clear TNF staining. Bright.
None
Recommend this antibody for flow analysis.