Marginal Zone B Cells In The Spleen

August 17, 2017

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

CD21/CD35 Monoclonal Antibody (eBio4E3 (4E3)), FITC

Catalog Number:

11-0212-82

The goal of the project was to determine the population of marginal zone B cells in the spleen after altering the expression of cytokine receptors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1: 100, 4 degrees, 30 min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Clearly marks marginal zone population (CD21hiCD23lo) when used in combination with CD23.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Good antibody for staining marginal zone B cells.

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