CD132 Staining Of B Cells

August 16, 2017

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Rat Anti-Mouse CD132

Catalog Number:

554457

The goal of the project was to determine the contribution of CD132 staining to germinal center B cell development. Also, to use this antibody for the staining of knockout cells once they are generated.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1: 50, 4 degrees, 30 min

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Good staining of CD132 expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, clearly marks positive cells

The Bad

None

The Bottom Line

Good antibody for staining CD132

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