CD4 PerCP-Cy5.5 Antibody

August 16, 2017

SNS
University of California, Merced
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience from Invitrogen

Product Name:

anti-mouse CD4 PerCP-Cy5.5

Catalog Number:

45-0042-82

I've been looking at different T cell populations and their activation. I've been using this CD4 antibody in all of my staining panels as it is one of the clearest and most consistent stains, even at a very low concentration.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymphocytes

Primary Incubation

1:1600 for 30min on ice

Blocking Agent

Fc Block or anti-CD16/32 blocking antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

Clearly distinguishes CD4 and CD8 T cell populations. Use it in every panel.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The high working dilution to save antibody and money

The Bad

None

The Bottom Line

Reliable stain and reasonable price given the antibody can be used so sparingly.

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