Promega
GoTaq® Green Master Mix
M7122
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We use this PCR master mix for genotyping PCR reaction for transgenic mice. We selected this product because of its ease of use, just add primers, water and template. It works well for our PCR reactions, is inexpensive and did not require much optimization.
PCR
Mouse genomic DNA
Follow the protocol, just add appropriate amounts of water, template and primers. Determine annealing temperature based on the melting temperature of your primers.
None
We consistently get strong bands with this master mix.
N/A
Reliable, easy to use
Great product, would recommend.