Anti-Mouse CD38

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-mouse CD38

Catalog Number:

102714

Based on the literature, CD38 can be used to differentiate between M1 and M2 macrophages. CD38 was used for this purpose. M1, M2, and M0 macrophage subsets were differentiated ex vivo from bone marrow cells and CD38 was used to confirm the population identity.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow derived cells

Primary Incubation

30 minutes on ice, 0.25ug per 100ul FACS buffer

Blocking Agent

FC block 1:100

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

The results show that none of my macrophage populations express CD38 or that the antibody is bad.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Does not stain

The Bottom Line

Need to find another clone or antibody to identify this cell subset.

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