Anti-Mouse CD64

August 14, 2017

Biology
Abbvie
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-mouse CD64 BV605

Catalog Number:

139323

General aim of the research is to quantify macrophages in the ex vivo prep of bone marrow derived cells. CD64 was used to be certain that the population generated are indeed macrophages.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow derived cells

Primary Incubation

30 minutes on ice, 0.5ug per 100ul facs buffer

Blocking Agent

FC block 1:100

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The results show that macrophages that I have generated in vitro are indeed macrophages based on CD64 and F4/80 staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Identifies the population of interest

The Bad

None

The Bottom Line

Good antibody for the purpose

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