BD Pharmingen
Purified Rat Anti-Mouse CD31 Clone MEC 13.3
557355
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When it is important to prove that the cells of interest are true endothelial cells or to visualize blood vessels in tissues, the researchers frequently use antibodies against the endothelial cell marker, CD31. We stained tumor-associated vasculature to analyze the structural differences in angiogenic blood vessels developing in the mice of different genetic background. We used rat anti-mouse CD31 antibody MEC 13.3 from BD Pharmingen to visualize tumor-associated vasculature in murine LLC primary tumors.
Immunohistochemistry
Frozen sections of primary tumor derived from Lewis lung carcinoma (LLC) cells
10 micrograms per ml PBS supplemented with 2% BSA; ON, 4oC
2% BSA in PBS
Goat anti-rat IgG conjugated with DyLight 488
NucBlue to stain cell nuclei
Immunofluorescence
The MEC 13.3 antibody nicely stains endothelial cells in the tumor-associated vessels.
N/A
Tissue sections should be frozen. We did not detect any CD31 in zinc-formalin-fixed paraffin sections.
The antibody is easy to use in IF applications.
None detected
The MEC 13.3 rat antibody is an excellent antibody for detection of murine CD31 in tumor-associated vessels.