Usage Of Anti CDC20 Antibody For Western Blots

August 08, 2017

chemical biology
indian institute of chemical technology

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Company:

Sigma

Product Name:

Anti-CDC20 (51-65) antibody

Catalog Number:

C5497

Browse Similar Products: CDC20 Western Blot Antibodies

The goal is to study the cell cycle regulation and associated molecular changes in G2/M boundary of cancer cells. The product was used to study the proteins functional modulation in cell cycle-dependent regulation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cell lysate

Primary Incubation

1:1000 dilution, at room temperature for 2hr

Blocking Agent

Skimmed milk

Secondary Incubation

1:5000 dilution, 1 hr at room temperature

Tertiary Incubation

Not required

Detection

Chemiluminescence

Results Summary

With the usage of antibody, we have observed CDC20 has not been interacting/regulating our protein of interest.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Antibody works well without any non specificity

The Bad

No negative remarks

The Bottom Line

Recommended for Immunoblot application

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