Anti-PE MicroBeads

University of Montana
DBS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-PE MicroBeads

Catalog Number:

130-048-801

We perform both depletion and selection of multiple cell types from cell cultures as well as whole tissues including spleen, lymph nodes, thymus, lung, and bone marrow. Cell samples are then used for RT-qPCR, Flow Cytometric analysis, and re-culturing for functional studies.

Experimental Design and Results Summary

Application

Cell selection or depletion

Starting Material

Mouse single cell suspension

Protocol Overview

Follow manufacture protocol for labeling cells with PE conjugated antibody. Follow with Anti-PE microbeads prior to addition to separation column or automatic separator.

Tips

You can use multiple PE conjugated antibodies to remove more than one cell type from samples

Results Summary

The anti-PE microbeads are versatile in their application and use for cell depletion/purification while still yielding highly pure samples.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Multiple uses

The Bad

None

The Bottom Line

We always have this kit around since PE is a very common fluorophore in our laboratory and we can customize panels of PE labeled cells for our desired purification.

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