Applied Biosystems by Thermo Fisher Scientific
SYBR Green PCR Master Mix
4309155
This master mix is used for standard qPCR assays. All components are premixed at working concentrations with the SYBR dye.
qPCR
cDNA and targer primer
Add cDNA, target primers and SYBR master mix to a qPCR plate. Run standard protocol on qPCR machine. Analyze data using delta CT method.
Try be as accurate as possible when pipetting
Ct values are obtained and can be used to calculate relative gene levels
N/A
Easy to use!
qPCR can be tricky and pippetting needs to be very accurate
A great master mix for standard qPCR assays