Isotype Control For PECy7 Labeled Antibodies

August 03, 2017

Pharmaceutical Technology
CIBER BBN
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Company:

Biolegend

Product Name:

PE/Cy7 Rat IgG2b, κ Isotype Ctrl Antibody

Catalog Number:

400617

We use this antibody as an isotype control for IgG2b, k antibodies labeled with PECy7 dye. It helps to discern unspecific signal from specific labeling.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspension from mouse spleen

Primary Incubation

1:100

Blocking Agent

We block with CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

With the channel for PECy7

Results Summary

It helps to discern the nonspecific signal obtained by IgG2b, k antibodies labeled with PECy7 from specific signal of the antibody. We use it to differentiate from the CD45 signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It is not expensive

The Bad

None

The Bottom Line

I recommend it for it rate quality/price

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