Effective Anti-Keratin 14 Antibody

July 31, 2017

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

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Company:

BioLegend

Product Name:

Purified anti-Keratin 14 Antibody

Catalog Number:

Poly9060

This chicken polyclonal antibody is used for the detection of Keratin 14. We used lysate from porcine dermal tissue to determine expression level. Combined with a DAB the antibody give a brown signal in epidermal cells. This antibody is widely published.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Paraffin embedded porcince skin tissues

Primary Incubation

1:100; 1 hour at room temperature

Blocking Agent

5% NGS

Secondary Incubation

1:500; biotinylated anti-chicken antibody 1 hour room temperature

Tertiary Incubation

Vector AB Kit; 30 minutes

Detection

Vector DAB kit SK4100

Results Summary

This effectively stained the keratin 14 cells within the epidermis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Effectively stained the protein of interest.

The Bad

None

The Bottom Line

Good antibody to stain keratin 14. Has the advantage of being raised in chicken, allowing it to be used in multiple stainings.

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