Western Blot Testing in Porcine Tissue

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

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Company:

Abcam

Product Name:

Rabbit polyclonal to Anti-MMP9 antibody

Catalog Number:

ab38898

This rabbit antibody is used for the detection of matrix metalloproteinase 9. We used lysate from porcine dermal tissue to determine expression level. While we were able to see clear bands control GAPDH bands we were not able to identify MMP9 bands. This antibody is widely published.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Porcine Tissue Lysate

Primary Incubation

1:1000 at 4 degrees overnight

Blocking Agent

5% milk in TBS-T

Secondary Incubation

1:2500 anti-rabbit HRP

Tertiary Incubation

None

Detection

ECL

Results Summary

No band was seen for MMP9 in pig tissue lysate.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Has been validated in other tissues.

The Bad

No band visible.

The Bottom Line

While this is a good antibody in other tissues, it is not suitable for pig tissue lysate.

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