IRDye® 800CW Goat anti-Rat IgG Review

July 25, 2017

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

LI-COR

Product Name:

IRDye® 800CW Goat anti-Rat IgG (H + L), 0.5 mg

Catalog Number:

926-32219

Our lab has generated mice that are infected with HIV at birth. We are interested at measuring HIV proteins different tissues to evaluate levels of infection and active replication. One of the detection methods is via western blot, and we use this antibody as secondary after the rat-anti-p24 antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse brain homogenate; mouse spleen homogenate

Primary Incubation

Rat anti-p24

Blocking Agent

5% milk, 5% goat serum

Secondary Incubation

Goat anti-rat

Tertiary Incubation

None

Detection

LI-COR Odyssey

Results Summary

Good detection; the levels sometimes are low so good antibodies are necessary

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear bands

The Bad

None

The Bottom Line

I recommend it

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