Good Antibody For Human B Cell Detection

July 28, 2017

Cell Biology
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

CD19 Monoclonal Antibody (HIB19), PE-Cyanine5

Catalog Number:

15-0199-42

The general aim of the research is to study hematopoiesis and acute myeloid leukemia. This antibody was used to identify and isolate B cells from human peripheral blood samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood

Primary Incubation

1:100

Blocking Agent

Fc block is added to cells prior to staining with antibody

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on a BD LSRII-yellow machine

Results Summary

This antibody gives a strong signal and clearly detects B cells from human blood

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong signal

The Bad

None

The Bottom Line

Recommended for isolation of human B cells

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