Invitrogen Antibodies
CD185 (CXCR5) Monoclonal Antibody (SPRCL5), Biotin, eBioscience™
13-7185-82
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Browse Similar Products: CD185 Flow Cytometry Antibodies
We work to identify the T follicular helper population in an autoimmune setting using this antibody for CXCR5 staining. Streptavidin conjugates provide the bright color we needed to identify the population while direct fluorophore conjugates didn't work as well for our experiments.
Flow Cytometry
Murine Lymphocytes
1:50 dilution, 1 hour at room temperature incubation
FC Block
1:100 Streptavidin dilution, 30 minutes at 4 degrees in the dark incubation
None
BD LSR II
CXCR5 on T cells appears as a branching population from the negative population. A fluorescence minus one can be used to detect the negative population.
N/A
It gives the bright signal needed to identify CXCR5.
It can be challenging to optimize.
You can identify CXCR5 more efficiently with this two-step staining method then with direct fluorophore conjugates.