Thermo Scientific
HOECHST 33342
62249
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We are studying how learning process is altered in a mouse model with cobalamin deficiency. To further understand it's underlying cause, we studied changes in the expression levels of AMPA receptors using immunofluorescence. We used HOECHT as a counter stain for this technique.
Immunohistochemistry
Paraffin embedded mouse brain sections
After incubation with secondary Ab, sections were washed in Modified Dulbecco's PBS containing 8mM sodium phosphate, 2mM potassium phosphate, 140mM sodium chloride, 10mM potassium chloride, pH 7.4. HOECHST staining was performed according to manufacturer's protocol.
Make sure to perform the counter-staining in dark
We got nuclei stained for the mouse sections (as shown in the image). It was helpful in locating hippocampus under a fluorescence microscope which was used to determine region specific expression of our protein of interest.
28545069
N/A
Easy to use and doesn't leak
Fades pretty fast
It is a good counter-stain and doesn't need any special treatment on the sections to make it work.