Vector Laboratories
HRP Goat Anti-Rabbit IgG Antibody (Peroxidase)
PI-1000
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We are studying a newly discovered receptor called transcobalamin receptor (TCblR) encoded by CD320 gene. This receptor is required for uptake of cobalamin in humans as well as mouse. To determine where it is expressed and how it is involved in materno-fetal transport of cobalamin, we studied its expression by immunohistochmeistry using this secondary Ab.
Immunohistochemistry
Paraffin embedded mouse placenta sections
1:200, 1 hour at room temperature
Normal goat serum
1:1000, 1 hr at room temperature
NA
DAB staining
We observed expression of TCblR on the fetal side of placenta, known as labyrinth. The expression was highly specific to this region in WT mouse. We used a TCblR KO mouse as a control and found no expression with immunostaining using the same protocol.
28351841
N/A
Works very well even at higher dilutions
Incubation has to be at least 1 hr
We have used this Ab with many other primary Abs for mouse sections it it always gives wonderful results.