Excellent For Paraffin Embedded Mouse Brain Tissues

July 13, 2017

Department of Cell Biology
SUNY Downstate Medical Center
PhD Candidate (Graduate Student)

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-PSD95 antibody

Catalog Number:

ab18258

We wanted to determine any changes in levels of PSD-95 in mouse hippocampus. We performed electrophysiology, particularly long term potentiation (LTP), and behavior studies on our KO mouse and observed some learning deficits. Our results showed no differences in the PSD-95 levels of WT and the KO mouse

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Paraffin embedded mouse brain

Primary Incubation

1:100, 22 hrs, 4 degree C

Blocking Agent

Normal goat serum

Secondary Incubation

1:200, 16 hrs, 4 degree C

Tertiary Incubation

None

Detection

Measured particles using fluorescence microscope

Results Summary

We found no significant differences in the PSD-95 levels (measured as number of particles counted per 100 um2 area).

DOI or PMID #

28545069

Additional Notes

N/A

Related Categories

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Summary

The Good

Works wonderfully for mouse tissues

The Bad

Incubation needs to be at least overnight

The Bottom Line

I like how it worked in one go for me. We were approaching the deadline for submission of our revised paper and the results from this Ab helped us further support our hypothesis.

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