Antibody To Detect Mature Murine Macrophages

July 17, 2017

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

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Company:

BioLegend

Product Name:

Alexa Fluor 488 anti-mouse F4/80 Antibody

Catalog Number:

123119

The rat mAb BM8 specifically recognizes the F4/80 antigen expressed on mature murine macrophages.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine bone marrow-derived macrophages

Primary Incubation

10 micrograms per ml PBS supplemented with 2% BSA

Blocking Agent

2% BSA in PBS

Secondary Incubation

NucBlue to highlight cell nuclei

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

The antibody specifically and with high sensitivity recognizes the antigen expressed on mature murine macrophages.

DOI or PMID #

N/A

Additional Notes

The antibody does not stain murine neutrophils.

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Summary

The Good

The antibody is easy to use in IF applications and flow cytometry.

The Bad

None detected

The Bottom Line

This antibody is a valuable tool in the analysis of murine macrophages and their discrimination from other blood cell types.

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