Anti Gr-1 Antibody

July 14, 2017

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

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Company:

BioLegend

Product Name:

Alexa Fluor 488 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Catalog Number:

108419

Rat mAb RB6-8C5 conjugated with Alexa Fluor 488 is a useful tool in detection of murine neutrophils (high levels of Gr-1), monocytes/macrophages (overall low levels of Gr-1), and immature myeloid derived cells (intermediate levels of Gr-1).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine peripheral blood

Primary Incubation

10 micrograms per ml PBS supplemented with 2% BSA

Blocking Agent

2% BSA in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

In peripheral blood, Gr-1 antibody (recognizing both Ly-6G and Ly-6C antigens) detects primarily mature neutrophils expressing high levels of Ly-6G antigen.

DOI or PMID #

N/A

Additional Notes

The image shows murine neutrophil (green staining), recognizable by its characteristic multi-lobulated nucleus

Related Categories

Image Gallery

Summary

The Good

The antibody is specific as it does not recognize mature monocytes in peripheral blood.

The Bad

None detected

The Bottom Line

The Gr-1 antibody RB6-8C5 is a valuable tool in detection of granulocytes in peripheral blood and in analysis of cell populations containing immature myeloid cells and neutrophils.

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