Rabbit antibody against iNOS (NOS2)

July 11, 2017

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz

Product Name:

N/A

Catalog Number:

sc-651

Rabbit antibody sc-651 detects murine and human iNOS with high specificity and sensitivity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates of murine macrophages

Primary Incubation

1 ug/ml in PBS supplemented with 5% nonfat milk and 0.05% Tween 20; overnight, cold room

Blocking Agent

5% nonfat milk in PBS/0.05% Tween 20

Secondary Incubation

Goat anti-rabbit IgG conjugated with HRP; 1:3,000; 1 hr, room temperature

Tertiary Incubation

None

Detection

West pico

Results Summary

This rabbit antibody detects murine (shown) and human (not shown) iNOS with high specificity and sensitivity. The antibody correctly detects iNOS in M1 macrophages, but not in M2 macrophages.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This antibody is highly specific and sensitive.

The Bad

None detected

The Bottom Line

This antibody is an excellent tool to detect iNOS in human and murine cells, including macrophages of M1 phenotype.

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