Review Of Anti-Ter119 BV510

July 10, 2017

Research
Bloodworks NW
Postdoctoral research fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells Antibody

Catalog Number:

116237

This antibody was chosen as a part of 8-color panel to assess the relative percentages of RBC vs. WBC.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Total mouse bone marrow without RBC lysis

Primary Incubation

30 mins @ +4C

Blocking Agent

anti-CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII

Results Summary

This antibody provided a good separation between positive and negative populations. Further discrimination between RBC and WBC can be made if you plot FSC-A vs. Ter119.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good separation between positive and negative

The Bad

None

The Bottom Line

Good choice of antibody for the BV510 fluorochrome.

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