Time Course Effect Of MCP1 On CCR4 Levels In HASMCs

July 13, 2017

Neurobiology
St. Jude Children's Research Hospital

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Sheep IgG HRP-conjugated Antibody

Catalog Number:

HAF016

HASMC lysates were analyzed for CCR4 levels using Western blot.Sheep IgG HRP-conjugated antibody was used as a secondary antibody in the blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human smooth muscle cell lysates

Primary Incubation

Anti-CCR4 antibody

Blocking Agent

5% skim milk powder

Secondary Incubation

Sheep IgG HRP-conjuaged antibody

Tertiary Incubation

None

Detection

Chemiluminescence

Results Summary

CCR4 levels are constant in human aortic smooth muscle cells upon MCP1 treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good antibody that was easy to optimize.

The Bad

Few non-specific bands are observed in the blot.

The Bottom Line

Good antibody that is publication quality!

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