Secondary Antibody Against Rabbit Primary for IF in AF488

July 13, 2017

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies by Thermo Fisher Scientific

 

Product Name:

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Catalog Number:

A-11034

We perform IF for cell counts and to measure MFI in tissue sections. This requires the use of secondaries in multiple colors to maximize the number of stains possible on a given sample.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain section

Primary Incubation

Anti-Myelin Basic Protein (Rabbit pAb)

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488

Tertiary Incubation

DAPI

Detection

Fluorescent microscope

Results Summary

Great staining with little to no background. Easy to visualize and quantify fluorescence.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining with little to no background

The Bad

None

The Bottom Line

Great secondary antibody for IF staining

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