eBioscience™ from Invitrogen Antibodies
PE-Cyanine7 Anti-Helios
25-9883-42
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Browse Similar Products: Helios Flow Cytometry Antibodies
I was interested in differentiating between thymus derived T-regs and peripherally derived T-regs and used Helios as a marker to determine this. This antibody was fantastic at detecting the presence of Helios in otherwise small populations of CD4+CD25+Foxp3+ cells and I'd highly recommend it!
Flow Cytometry
Isolated mouse CD4 T-cells induced in vitro
3 hour fix perm buffer
FC block
1:200 dilution, 30 min at 4 C
None
Flow Cytometer
Clear distinction between nTregs and iTregs. Would definitely use again and I highly recommend.
N/A
Good quality data, reliable
Worth the price and eBioscience is an excellent company for detecting these intracellular markers.