Good PDGFR Antibody for Immunohistochemistry

July 03, 2017

Pathology
Albert Einstein College of Medicine
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human PDGF R alpha Antibody

Catalog Number:

AF-307-NA

We wanted to compare the amount PDGFR positive cells in the spinal cords of 2 different mouse strains.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse spinal cord

Primary Incubation

3ug/ml dilition overnight at 4 degrees celcius

Blocking Agent

5% Goat Serum in %5 Milk

Secondary Incubation

Anti-goat-HRP (1:500) secondary antibody for 1 hour at room temperature

Tertiary Incubation

None

Detection

DAB

Results Summary

We were able to detect significant differences in the number of PDGFR positive cells between our mouse strains

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Low Backgound

The Bad

None

The Bottom Line

Great for detecting PDGFR in mouse brain

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