Isotype Control For PE/Cy7 Flurophore

July 07, 2017

Center for Regenerative Medicine
Boston University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cy7 Rat IgG2a, κ Isotype Ctrl Antibody

Catalog Number:

400521

The aim of this experiment was to use the PE/Cy7 isotype as a negative control for sorting PE/Cy7 CD31 positive cells in the mouse lung. This product has been recommended by the manufacturer as the optimal isotype for PE/Cy7.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

1:80

Blocking Agent

2% FBS in HBSS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

PE/Cy7 was detected using FACS

Results Summary

The isotype control worked as expected. It was possible to use it as the negative control for gating PE/Cy7 CD31 positive cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to optimize, stains as expected.

The Bad

None

The Bottom Line

Set the dilution of the isotype control the same as the antibody used for staining the specific cell population.

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