Biolegend
PE/Cy7 anti-mouse CD31 Antibody
102417
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The aim of the experiment was to stain the endothelium of mouse lungs using the CD-31 antibody, as endothelial cells express it. This antibody has been cited in the literature, hence why we used it in our experiment.
Flow Cytometry
Harvested mouse lung cells
1:80
2% FBS in HBSS
N/A
PE/Cy7 was detected using FACS
The percentage of cells positive for CD31 were determined when sorting the cells. The antibody stained the epithelium as expected.
Easy to optimize, stains as expected.
None
Stains the epithelium as reported in the literature.