Biolegend
Mouse IL-6 ELISA MAX™ Standard
431302
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The kit is specially designed for performing ELISA on mouse serum and cell culture supernatants. It is very useful when you have a large number of samples and is very cost efficient and easy to use.
ELISA
Mouse serum and supernatants from bone marrow derived macrophages
Pre-working day (24h before working).Dilute your Capture antibody in the Coating buffer and add 100 microliter/well to the 96 well/plate.Next day, Wash plate and block it for 1 hour with Assay diluent. After the blocking time wash and add your samples and standards and incubate for 2h in RT (room temperature). After 2h of incubation wash again and add the diluted Detection antibody (incubate for 1h). Then wash again and add the enzyme (diluted in Assay diluent) and incubate for 30 min., then wash again and add the TMB Substrate solution. In the end use the Stop solution. Read the plate in a plate reader
Best when run your samples fresh.
Was able to detect levels of cytokines reliably in serum and cell culture supernatants.
N/A
Very reliable and easy to use.
Needs a big sample volume
Is highly easy to use, and very reliable. Also was cheap to perform when you are running a large number of samples.