Excellent Antibody Against Murine TIMP-1

June 27, 2017

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Goat anti Mouse TIMP-1 Antibody

Catalog Number:

AF980

The AF980 goat polyclonal antibody AF98- from R&D Systems can be successfully used to detect murine TIMP-1 by western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Recombinant murine TIMP-1

Primary Incubation

1 ug/ml in PBS supplemented with 5% nonfat milk and 0.05% Tween 20; overnight, cold room

Blocking Agent

5% nonfat milk in PBS/0.05% Tween 20

Secondary Incubation

Donkey anti-goat HRP-conjugated antibody

Tertiary Incubation

None

Detection

West pico

Results Summary

This anti-murine TIMP-1 antibody detects low ng quantities of recombinant TIMP-1 in western blotting procedures. The image shows the reliable detection of ~28 kDa recombinant TIMP-1 protein loaded at 1 and 3 ng per lane.

DOI or PMID #

N/A

Additional Notes

This antibody also recognises natural TIMP-1 produced by murine normal and tumor cells.

Related Categories

Image Gallery

Summary

The Good

High sensitivity and specificity

The Bad

None detected

The Bottom Line

This is an excellent antibody to detect murine TIMP-1.

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