Low Mol. Wt. (70 KDa) Rhodamine-Conjugated Dextran For Vascular Biology Research

June 28, 2017

The Scripps Research Institute
The Cell and Molecular Biology
Assistant Professor

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Company:

Sigma Aldrich

Product Name:

Rhodamine B isothiocyanate-Dextran

Catalog Number:

R9379

The low mol wt. Rhodamine-Dextran is used to highlight normal vasculature in live animals.

Experimental Design and Results Summary

Application

Immunofluorescent staining of normal vasculature

Starting Material

Chick embryos incubated for 12-15 days.

Protocol Overview

Dextran is used at concentration 0.5-1 mg/ml in PBS; injected 0.1-0.15 ml per embryo; the vasculature is highlighted within several minutes and can be visualized in immunofluorescent microscope without any fixation.

Tips

The time for incubation should be short (minutes) since this low. mol. dextran will leak even from normal blood vessels.

Results Summary

This dextran is excellent reagent to stain normal vasculature in chick embryos.

DOI or PMID #

N/A

Additional Notes

It is possible to use this dextran in vessel permeability research, but the time and concentration should be carefully adjusted.

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Summary

The Good

This dextran is suitable for staining of animal vasculature in vivo.

The Bad

This dextran could be too "leaky" and produce non-specific staining around normal blood vessels.

The Bottom Line

This red fluorescent dextran is suitable for staining of normal blood vessels and could be adjusted for the use in vessel permeability studies.

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