Sigma Aldrich
Fluorescein isothiocyanate-dextran
FD2000S
FD2000S is a FITC-conjugated, high mol. wt., non-permeable dextran used in vascular research to label endothelial cells in vivo or fill blood vessels to measure volume of perfusable vasculature.
Immunofluorescent staining of the vasculature
Live chick embryos
The chick embryos at day 12-16 incubation are inoculated i.v. with FITC-dextran and the vasculature could be vizualized in immunofluorescent microscope within several minutes.
The low concentrations of dextran could be used for simple labeling of the vasculature, whereas higher concentrations (e.g. 1-5 mg/ml) are suitable to measure the volume of perfusable vasculature.
The FD2000S FITC-dextran (mol. wt. 2,000 kDa) is an excellent agent to label the vasculature of chick embryos.
N/A
In combination with low mol. wt. red fluorescent dextran, the FICT-conjugated high mol. wt. dextran can be used to measure the permeability of the vasculature, e.g. tumor vasculature.
This dextran has very low level of permeability, i.e. does not leak from normal blood vessels.
None detected
The FD2000S Dextran is an excellent tool to label in vivo the perfusable vasculature.