Excellent Anti-human MMP-1 Antibody for Western Blotting

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human MMP-1 Antibody

Catalog Number:

MAB901

The murine monoclonal IgG1 antibody MAB901 against human MMP-1 nicely recognizes recombinant MMP-1 protein (zymogen and activated enzyme) as well as MMP-1 produced by various tumor cells, including epidermoid carcinoma cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human tumor cells

Primary Incubation

1 ug/ml in PBS supplemented with 5% nonfat milk and 0.05% Tween 20; overnight, cold room

Blocking Agent

5% nonfat milk in PBS/0.05% Tween 20

Secondary Incubation

Goat anti-mouse IgG conjugated with HRP; 1:3,000; 1 hr, room temperature

Tertiary Incubation

None

Detection

West pico

Results Summary

The MAB901 antibody reliably recognizes the zymogen of MMP-1 (~50-52-kDa) and activated enzyme species (~44-46 kDa).

DOI or PMID #

10.1158/0008-5472.CAN-12-4495

Additional Notes

The antibody can also be used in immunohistochemistry.

Related Categories

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Summary

The Good

The antibody MAB901 is sensitive and specific.

The Bad

None detected

The Bottom Line

The antibody MAB901 is sensitive, specific and reliable in western blot applications for detection of human MMP-1.

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